||Recombinant Human SUMO1 (Accession # NM_003352) , fused with an N-terminal 6-His tag, was produced in E. coli-derived.
||Supplied as a solution in HEPES, NaCl and DTT.
||Recombinant Human His6-Pro-SUMO1 can be conjugated to substrate proteins via the subsequent actions of an SUMO-activating (E1) enzyme, an SUMO-conjugating (E2) enzyme, and an SUMO ligase (E3). Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-Pro-SUMO1 concentration of 10-50 µM.
||>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
||Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).