||Recombinant Human SUMO1 (Accession # NM_003352) was produced in E. coli-derived.
||Supplied as a solution in HEPES, NaCl and DTT
||SUMO1 chains do not readily form in vitro . However, the role of SUMO1 in poly-SUMO chain formation is an area of intense research. Utilizing Recombinant Human SUMO1 Mutant K7R/K16R will ensure that K7 and K16-linked chains will not be formed. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human SUMO1 Mutant K7R/K16R concentration of 10-50 µM.
||>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
||Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).