Recombinant Human SEMA5B, Fc Chimera SEMA5B-652H

Recombinant Human SEMA5B, Fc Chimera

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Recombinant Human SEMA5B, Fc Chimera

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Cat.No. : SEMA5B-652H
Product Overview : Recombinant Human SEMA5B (Accession # NP_001026872) was produced in Chinese Hamster Ovary cell line, CHO-derived.
Source : CHO cells
Species : Human
Tag : Fc Chimera
Predicted N Terminal : Val78
Form : Lyophilized from a 0.2 µm filtered solution in PBS.
Bio-activity : Measured by the ability of the immobilized protein to support the adhesion of human pancreatic cancer cells.When 5 x 10^4 cells/well are added to Recombinant Human Semaphorin 5B Fc Chimera coated plates, cell adhesion is enhanced in a dose dependent manner after 1 hour at 37 °C. The ED50 for this effect is typically 0.7 - 2.8 µg/mL.
Molecular Mass : Recombinant Human SEMA5B, Fc Chimera has a calculated MW of 110.3 kDa (monomer). In SDS-PAGE migrates as 130-140 kDa, reducing conditions.
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name : SEMA5B sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B [ Homo sapiens ]
Official Symbol : SEMA5B
Synonyms : SemG; SEMAG
Gene ID : 54437
mRNA Refseq : NM_001256348.1
Protein Refseq : NP_001243277.1
MIM : 609298
UniProt ID : Q9P283
Chromosome Location : 3q21.1

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