Recombinant Human NCAM1 NCAM1-660H

Recombinant Human NCAM1

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Recombinant Human NCAM1

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Cat.No. : NCAM1-660H
Product Overview : Recombinant Human NCAM1 (Accession # NP_001070150) was produced in Mouse myeloma cell line, NS0-derived.
Source : NSO cells
Species : Human
Predicted N Terminal : Leu20
Form : Lyophilized from a 0.2 µm filtered solution in Tris and NaCl.
Bio-activity : Measured by the ability of the immobilized protein to support the adhesion of Neuro-2A mouse neuroblastoma cells.When 5 x 10^4 cells/well are added to recombinant human NCAM-1 coated plates, cell adhesion is enhanced in a dose dependent manner after 1 hour incubation at 37 °C. The ED50 for this effect is typically 0.5 - 3 µg/mL. Optimal dilutions should be determined by each laboratory for each application.
Molecular Mass : Recombinant Human NCAM1 has a calculated MW of 76.5 kDa. In SDS-PAGE migrates as 120 kDa, reducing conditions.
Purity : >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name : NCAM1 neural cell adhesion molecule 1 [ Homo sapiens ]
Official Symbol : NCAM1
Synonyms : NCAM1; neural cell adhesion molecule 1; CD56; NCAM; neural cell adhesion molecule, NCAM; antigen recognized by monoclonal 5.1H11; MSK39;
Gene ID : 4684
mRNA Refseq : NM_000615
Protein Refseq : NP_000606
MIM : 116930
UniProt ID : P13591
Chromosome Location : 11q23.2

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