Active Recombinant Human B4GALT1 Protein (Y285L, Gly44-Ser398), N-6×His tagged

• Cat.No.: B4GALT1-716H
• Product Overview: Recombinant Human B4GalT1 Protein (Y285L, Gly44-Ser398), with an N-terminal 6-His tag was expressed in Mouse myeloma cell line.

Specification

• Description: This gene is one of seven beta-1, 4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1, 4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. This gene is unique among the beta4GalT genes because it encodes an enzyme that participates both in glycoconjugate and lactose biosynthesis. For the first activity, the enzyme adds galactose to N-acetylglucosamine residues that are either monosaccharides or the nonreducing ends of glycoprotein carbohydrate chains. The second activity is restricted to lactating mammary tissues where the enzyme forms a heterodimer with alpha-lactalbumin to catalyze UDP-galactose + D-glucose < => UDP + lactose. The two enzymatic forms result from alternate transcription initiation sites and post-translational processing. Two transcripts, which differ only at the 5' end, with approximate lengths of 4.1 kb and 3.9 kb encode the same protein. The longer transcript encodes the type II membrane-bound, trans-Golgi resident protein involved in glycoconjugate biosynthesis. The shorter transcript encodes a protein which is cleaved to form the soluble lactose synthase.
• Source: Mouse myeloma cell line
• Species: Human
• Tag: N-6×His
• Bio-activity: Measured by its ability to transfer N-Acetyl-D-galactosamine from UDP-GalNAc to N-Acetyl-D-glucosamine. The specific activity is > 2, 000 pmol/min/μg
• Molecular Mass: Predicted Molecular Mass: 40 kDa
  SDS-PAGE: 45-60 kDa, reducing conditions
• Protein Length: (Y285L, Gly44-Ser398)
• N-terminal Sequence Analysis: His
• Endotoxin: < 1.0 EU/μg of the protein by the LAL method.
• Purity: > 95 % by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -20 to -70 centigrade as supplied.
  3 months, -20 to -70 centigrade under sterile conditions after opening.
• Storage Buffer: Supplied as a 0.2 μm filtered solution in Tris, NaCl and TCEP.
• Shipping: The product is shipped with polar packs.

Gene Information

• Gene Name: B4GALT1 beta-1, 4-galactosyltransferase 1 [ Homo sapiens (human) ]
• Official Symbol: B4GALT1
• Synonyms: B4GALT1; beta-1, 4-galactosyltransferase 1; GT1; GTB; CDG2D; GGTB2; CLDLFIB; B4GAL-T1; beta4Gal-T1; beta-1, 4-galactosyltransferase 1; N-acetyllactosamine synthase; UDP-Gal:beta-GlcNAc beta-1, 4-galactosyltransferase 1; UDP-Gal:betaGlcNAc beta 1, 4- galactosyltransferase, polypeptide 1; UDP-galactose:beta-N-acetylglucosamine beta-1, 4-galactosyltransferase 1; beta-1, 4-GalTase 1; beta-N-acetylglucosaminyl-glycolipid beta-1, 4-galactosyltransferase; beta-N-acetylglucosaminylglycopeptide beta-1, 4-galactosyltransferase; glycoprotein-4-beta-galactosyltransferase 2; lactose synthase A protein; nal synthase; neolactotriaosylceramide beta-1, 4-galactosyltransferase; EC 2.4.1.22; EC 2.4.1.275; EC 2.4.1.38; EC 2.4.1.90
• Gene ID: 2683
• mRNA Refseq: NM_001497
• Protein Refseq: NP_001488
• MIM: 137060
• UniProt ID: P15291