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Recombinant Human CTSE, His-tagged

Cat.No.: CTSE-724H
Product Overview: Recombinant Human CTSE (Accession # NP_001901) Gln18-Pro396, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Source: NSO cells
Species: Human
Tag: His
Predicted N Terminal: Ile54 & Gln18 (predicted)
Form: Lyophilized from a 0.2 µm filtered solution in MES and NaCl.
Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2 .The specific activity is >1,500 pmol/min/µg, as measured under the described conditions.
Molecular Mass: Recombinant Human CTSE, His-tagged has a calculated MW of 42 kDa. In SDS-PAGE migrates as 48 kDa, reducing conditions.
Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name: CTSE cathepsin E [ Homo sapiens ]
Official Symbol: CTSE
Synonyms: CTSE; cathepsin E; slow-moving proteinase; erythrocyte membrane aspartic proteinase; CATE;
Gene ID: 1510
mRNA Refseq: NM_001910
Protein Refseq: NP_001901
MIM: 116890
UniProt ID: P14091
Chromosome Location: 1q32.1

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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