Active Recombinant Human IGFBP6, Met-tagged

• Cat.No.: IGFBP6-735H
• Product Overview: Recombinant Human IGFBP6 (Accession # NP_002169) Ala25-Gly240, fused with an N-terminal Met, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Predicted N Terminal: Met
• Form: Lyophilized from a 0.2 µm filtered solution in Acetonitrile and TFA.
• Bio-activity: Measured by its ability to inhibit the biological activity of IGF-I or IGF-II on MCF-7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083.The ED50 for this effect is typically 0.1 - 0.4 µg/mL in the presence of 14 ng/mL rhIGF-II.
• Molecular Mass: Recombinant Human IGFBP6, Met-tagged has a calculated MW of 23 kDa. In SDS-PAGE migrates as 34 kDa, reducing conditions.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: IGFBP6 insulin-like growth factor binding protein 6 [ Homo sapiens ]
• Official Symbol: IGFBP6
• Synonyms: IGFBP6; insulin-like growth factor binding protein 6; insulin-like growth factor-binding protein 6; IBP-6; IGFBP-6; IGF binding protein 6; IGF-binding protein 6; IBP6
• Gene ID: 3489
• mRNA Refseq: NM_002178
• Protein Refseq: NP_002169
• MIM: 146735
• UniProt ID: P24592
• Chromosome Location: 12q13