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Active Recombinant Human PAK1, GST-tagged

Cat.No. : PAK1-1259H
Product Overview : Recombinant Rac/Cdc42 (p21) binding domain (PBD) of the human p21 activated kinase 1 protein (PAK) protein has been expressed as a GST-fusion protein in E. coli
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Description : Serine/threonine-protein kinase PAK 1 is an enzyme that in humans is encoded by the PAK1 gene.
Source : E. coli
Species : Human
Tag : GST
Form : lyophilized. 50 mM Tris pH 7.5, 50 mM NaCl, 1.0% (w/v) dextran and 5% (w/v) sucrose.
Bio-activity : The PAK-PBD protein specifically recognizes and binds to the active, GTP-bound, form of Rac and Cdc42. Biological activity of PAK-PBD protein is therefore tested by its selectivity for GTPγS loaded Rac and Cdc42 proteins.
Molecular Mass : 34 kDa
Protein length : 67-150
Purity : rotein purity is determined by scanning densitometry of Coomassie Blue stained protein on a 12% SDS polyacrylamide gel. GST-PAK-PBD protein is >80% pure.
Stability : The lyophilized protein is stable for 6 months if stored desiccated to <10% humidity at 4 centigrade.
Storage : Briefly centrifuge to collect the product at the bottom of the tube. The protein should be reconstituted to 3 mg/ml by the addition of 83 µl of distilled water. For storage, the reconstituted protein should be aliquoted into experiment sized amounts, snap frozen in liquid nitrogen and stored at -70 centigrade. Under these conditions the protein is stable for 6 months. To maintain high biological activity, the protein should not be exposed to repeated freeze thaw cycles.
Gene Name : PAK1 p21 protein (Cdc42/Rac)-activated kinase 1 [ Homo sapiens ]
Official Symbol : PAK1
Synonyms : serine/threonine-protein kinase PAK 1; STE20 homolog, yeast; alpha-PAK; p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast); p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related); p65-PAK
Gene ID : 5058
mRNA Refseq : NM_001128620
Protein Refseq : NP_001122092
MIM : 602590
UniProt ID : Q13153
Chromosome Location : 11q13-q14
Pathway : Activation of Rac, organism-specific biosystem; Adaptive Immune System, organism-specific biosystem; Axon guidance, organism-specific biosystem
Function : ATP binding; collagen binding; protein binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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What role does PAK1 play in cancer progression and metastasis? 01/16/2022

PAK1 has been implicated in cancer progression due to its roles in cell survival, motility, and angiogenesis. Its overexpression has been observed in various tumors and is often associated with increased invasiveness and poor prognosis.

Are there any therapeutic implications related to targeting PAK1 in diseases? 07/17/2021

Given PAK1's involvement in cancer progression and other diseases, it has emerged as a potential therapeutic target. Several PAK1 inhibitors are under investigation for their potential in cancer therapy and other conditions where PAK1 signaling is dysregulated.

What are the downstream targets of PAK1 in signal transduction? 11/10/2018

Once activated, PAK1 phosphorylates a variety of substrates, including kinases, cytoskeletal proteins, and transcription factors. Some of its known targets include Raf-1, MEK1, and LIM kinases.

What is the primary function of the PAK1 protein in cellular signaling? 10/23/2018

PAK1 is a serine/threonine kinase that plays a crucial role in multiple cellular processes, including cytoskeletal reorganization, cell motility, and cell survival, primarily through its role in various signaling pathways.

How does PAK1 contribute to cytoskeletal dynamics? 06/17/2018

PAK1 regulates cytoskeletal dynamics by phosphorylating and modulating the activity of several cytoskeletal proteins, including filamin, LIM kinases, and myosin light chain, thereby influencing cell shape, motility, and adhesion.

How is PAK1 activated in cells? 01/24/2018

PAK1 is activated through binding to the GTP-bound forms of Cdc42 or Rac, small Rho GTPases. This binding induces a conformational change in PAK1, leading to its autophosphorylation and activation.

Are there any known genetic mutations in the PAK1 gene associated with diseases? 01/01/2018

While mutations in PAK1 are not commonly associated with hereditary diseases, its overexpression or hyperactivation has been linked to various cancers, promoting cell proliferation, survival, and migration.

Customer Reviews (3)

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Reviews
01/07/2020

    Worked as expected, perfect.

    01/23/2019

      Quick and efficient delivery.

      01/18/2018

        High purity, excellent yield.

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