Active Recombinant Human PAK1, GST-tagged
Cat.No. : | PAK1-1259H |
Product Overview : | Recombinant Rac/Cdc42 (p21) binding domain (PBD) of the human p21 activated kinase 1 protein (PAK) protein has been expressed as a GST-fusion protein in E. coli |
- Specification
- Gene Information
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Description : | Serine/threonine-protein kinase PAK 1 is an enzyme that in humans is encoded by the PAK1 gene. |
Source : | E. coli |
Species : | Human |
Tag : | GST |
Form : | lyophilized. 50 mM Tris pH 7.5, 50 mM NaCl, 1.0% (w/v) dextran and 5% (w/v) sucrose. |
Bio-activity : | The PAK-PBD protein specifically recognizes and binds to the active, GTP-bound, form of Rac and Cdc42. Biological activity of PAK-PBD protein is therefore tested by its selectivity for GTPγS loaded Rac and Cdc42 proteins. |
Molecular Mass : | 34 kDa |
Protein length : | 67-150 |
Purity : | rotein purity is determined by scanning densitometry of Coomassie Blue stained protein on a 12% SDS polyacrylamide gel. GST-PAK-PBD protein is >80% pure. |
Stability : | The lyophilized protein is stable for 6 months if stored desiccated to <10% humidity at 4 centigrade. |
Storage : | Briefly centrifuge to collect the product at the bottom of the tube. The protein should be reconstituted to 3 mg/ml by the addition of 83 µl of distilled water. For storage, the reconstituted protein should be aliquoted into experiment sized amounts, snap frozen in liquid nitrogen and stored at -70 centigrade. Under these conditions the protein is stable for 6 months. To maintain high biological activity, the protein should not be exposed to repeated freeze thaw cycles. |
Gene Name : | PAK1 p21 protein (Cdc42/Rac)-activated kinase 1 [ Homo sapiens ] |
Official Symbol : | PAK1 |
Synonyms : | serine/threonine-protein kinase PAK 1; STE20 homolog, yeast; alpha-PAK; p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast); p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related); p65-PAK |
Gene ID : | 5058 |
mRNA Refseq : | NM_001128620 |
Protein Refseq : | NP_001122092 |
MIM : | 602590 |
UniProt ID : | Q13153 |
Chromosome Location : | 11q13-q14 |
Pathway : | Activation of Rac, organism-specific biosystem; Adaptive Immune System, organism-specific biosystem; Axon guidance, organism-specific biosystem |
Function : | ATP binding; collagen binding; protein binding |
Products Types
◆ Recombinant Protein | ||
PAK1-5512H | Recombinant Human Protein (Cdc42/Rac)-Activated Kinase 1, GST-tagged | +Inquiry |
PAK1-768H | Recombinant Human PAK1 Protein, His-tagged | +Inquiry |
Pak1-4662M | Recombinant Mouse Pak1 Protein, Myc/DDK-tagged | +Inquiry |
PAK1-3921R | Recombinant Rat PAK1 Protein, His (Fc)-Avi-tagged | +Inquiry |
PAK1-17H | Active Recombinant Human PAK1 | +Inquiry |
◆ Lysates | ||
PAK1-1275HCL | Recombinant Human PAK1 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionPAK1 has been implicated in cancer progression due to its roles in cell survival, motility, and angiogenesis. Its overexpression has been observed in various tumors and is often associated with increased invasiveness and poor prognosis.
Given PAK1's involvement in cancer progression and other diseases, it has emerged as a potential therapeutic target. Several PAK1 inhibitors are under investigation for their potential in cancer therapy and other conditions where PAK1 signaling is dysregulated.
Once activated, PAK1 phosphorylates a variety of substrates, including kinases, cytoskeletal proteins, and transcription factors. Some of its known targets include Raf-1, MEK1, and LIM kinases.
PAK1 is a serine/threonine kinase that plays a crucial role in multiple cellular processes, including cytoskeletal reorganization, cell motility, and cell survival, primarily through its role in various signaling pathways.
PAK1 regulates cytoskeletal dynamics by phosphorylating and modulating the activity of several cytoskeletal proteins, including filamin, LIM kinases, and myosin light chain, thereby influencing cell shape, motility, and adhesion.
PAK1 is activated through binding to the GTP-bound forms of Cdc42 or Rac, small Rho GTPases. This binding induces a conformational change in PAK1, leading to its autophosphorylation and activation.
While mutations in PAK1 are not commonly associated with hereditary diseases, its overexpression or hyperactivation has been linked to various cancers, promoting cell proliferation, survival, and migration.
Customer Reviews (3)
Write a reviewWorked as expected, perfect.
Quick and efficient delivery.
High purity, excellent yield.
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