Recombinant Human PAK1, GST-tagged


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Recombinant Human PAK1, GST-tagged

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Cat.No. : PAK1-1259H
Product Overview : Recombinant Rac/Cdc42 (p21) binding domain (PBD) of the human p21 activated kinase 1 protein (PAK) protein has been expressed as a GST-fusion protein in E. coli
Description : Serine/threonine-protein kinase PAK 1 is an enzyme that in humans is encoded by the PAK1 gene.
Source : E. coli
Species : Human
Tag : GST
Form : lyophilized. 50 mM Tris pH 7.5, 50 mM NaCl, 1.0% (w/v) dextran and 5% (w/v) sucrose.
Bio-activity : The PAK-PBD protein specifically recognizes and binds to the active, GTP-bound, form of Rac and Cdc42. Biological activity of PAK-PBD protein is therefore tested by its selectivity for GTPγS loaded Rac and Cdc42 proteins.
Molecular Mass : 34 kDa
Protein length : 67-150
Purity : rotein purity is determined by scanning densitometry of Coomassie Blue stained protein on a 12% SDS polyacrylamide gel. GST-PAK-PBD protein is >80% pure.
Stability : The lyophilized protein is stable for 6 months if stored desiccated to <10% humidity at 4 centigrade.
Storage : Briefly centrifuge to collect the product at the bottom of the tube. The protein should be reconstituted to 3 mg/ml by the addition of 83 µl of distilled water. For storage, the reconstituted protein should be aliquoted into experiment sized amounts, snap frozen in liquid nitrogen and stored at -70 centigrade. Under these conditions the protein is stable for 6 months. To maintain high biological activity, the protein should not be exposed to repeated freeze thaw cycles.
Gene Name : PAK1 p21 protein (Cdc42/Rac)-activated kinase 1 [ Homo sapiens ]
Official Symbol : PAK1
Synonyms : serine/threonine-protein kinase PAK 1; STE20 homolog, yeast; alpha-PAK; p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast); p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related); p65-PAK
Gene ID : 5058
mRNA Refseq : NM_001128620
Protein Refseq : NP_001122092
MIM : 602590
UniProt ID : Q13153
Chromosome Location : 11q13-q14
Pathway : Activation of Rac, organism-specific biosystem; Adaptive Immune System, organism-specific biosystem; Axon guidance, organism-specific biosystem
Function : ATP binding; collagen binding; protein binding
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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