Active Recombinant Human PAK1, GST-tagged

• Cat.No.: PAK1-1259H
• Product Overview: Recombinant Rac/Cdc42 (p21) binding domain (PBD) of the human p21 activated kinase 1 protein (PAK) protein has been expressed as a GST-fusion protein in E. coli

Specification

• Description: Serine/threonine-protein kinase PAK 1 is an enzyme that in humans is encoded by the PAK1 gene.
• Source: E. coli
• Species: Human
• Tag: GST
• Form: lyophilized. 50 mM Tris pH 7.5, 50 mM NaCl, 1.0% (w/v) dextran and 5% (w/v) sucrose.
• Bio-activity: The PAK-PBD protein specifically recognizes and binds to the active, GTP-bound, form of Rac and Cdc42. Biological activity of PAK-PBD protein is therefore tested by its selectivity for GTPγS loaded Rac and Cdc42 proteins.
• Molecular Mass: 34 kDa
• Protein length: 67-150
• Purity: rotein purity is determined by scanning densitometry of Coomassie Blue stained protein on a 12% SDS polyacrylamide gel. GST-PAK-PBD protein is >80% pure.
• Stability: The lyophilized protein is stable for 6 months if stored desiccated to <10% humidity at 4 centigrade.
• Storage: Briefly centrifuge to collect the product at the bottom of the tube. The protein should be reconstituted to 3 mg/ml by the addition of 83 µl of distilled water. For storage, the reconstituted protein should be aliquoted into experiment sized amounts, snap frozen in liquid nitrogen and stored at -70 centigrade. Under these conditions the protein is stable for 6 months. To maintain high biological activity, the protein should not be exposed to repeated freeze thaw cycles.

Gene Information

• Gene Name: PAK1 p21 protein (Cdc42/Rac)-activated kinase 1 [ Homo sapiens ]
• Official Symbol: PAK1
• Synonyms: serine/threonine-protein kinase PAK 1; STE20 homolog, yeast; alpha-PAK; p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast); p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related); p65-PAK
• Gene ID: 5058
• mRNA Refseq: NM_001128620
• Protein Refseq: NP_001122092
• MIM: 602590
• UniProt ID: Q13153
• Chromosome Location: 11q13-q14
• Pathway: Activation of Rac, organism-specific biosystem; Adaptive Immune System, organism-specific biosystem; Axon guidance, organism-specific biosystem
• Function: ATP binding; collagen binding; protein binding