Active Recombinant Human PRSS8 Protein, His-tagged

• Cat.No.: PRSS8-518H
• Product Overview: Recombinant Human PRSS8 (Accession # NP_002764) Ala33-Gly319, fused with a C-terminal 10-His tag, was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Source: CHO Cells
• Species: Human
• Tag: His
• Predicted N Terminal: Ala33 & Ile45
• Form: Supplied as a 0.2 µm filtered solution in Tris, NaCl and CaCl2.
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate Boc-QAR-AMC.The specific activity is >10 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Human PRSS8, His-tagged has a calculated MW of 32 kDa (Pro) & 31 kDa (Mature). In SDS-PAGE migrates as 39 kDa, reducing conditions.
• Protein length: Ala33-Gly319
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: PRSS8 protease, serine, 8 [ Homo sapiens ]
• Official Symbol: PRSS8
• Synonyms: PRSS8; protease, serine, 8; prostasin; serine protease 8; channel-activating protease 1; CAP1; PROSTASIN
• Gene ID: 5652
• mRNA Refseq: NM_002773
• Protein Refseq: NP_002764
• MIM: 600823
• UniProt ID: Q16651
• Chromosome Location: 16p11.2