Active Recombinant Human SLIT1 Protein, His-tagged

• Cat.No.: SLIT1-524H
• Product Overview: Recombinant Human SLIT1 (Accession # NP_003052) Met1-Gln915, fused with a C-terminal 6-His tag, was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Source: CHO Cells
• Species: Human
• Tag: His
• Predicted N Terminal: Cys34
• Form: Lyophilized from a 0.2 µm filtered solution in MOPS and NaCl.
• Bio-activity: Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons.Optimal neurite outgrowth was observed when neurons were plated on 96-well plates that had been pre-coated with 50 µL/well of Recombinant Human Slit1 at 2.5 - 10 µg/mL.
• Molecular Mass: Recombinant Human SLIT1, His-tagged has a calculated MW of 98.8 kDa. In SDS-PAGE migrates as 105-125 kDa, reducing conditions.
• Protein length: Met1-Gln915
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: SLIT1 slit homolog 1 (Drosophila) [ Homo sapiens ]
• Official Symbol: SLIT1
• Synonyms: SLIT1; slit homolog 1 (Drosophila); SLIL1, slit (Drosophila) homolog 1; slit homolog 1 protein; MEGF4; Slit 1; slit1; SLIT3; multiple EGF-like domains protein 4; multiple epidermal growth factor-like domains protein 4; SLIL1; SLIT-1; MGC164811
• Gene ID: 6585
• mRNA Refseq: NM_003061
• Protein Refseq: NP_003052
• MIM: 603742
• UniProt ID: O75093
• Chromosome Location: 10q23.3-q24