Recombinant Human UBE2B Protein, His-tagged UBE2B-531H

Recombinant Human UBE2B Protein, His-tagged

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Recombinant Human UBE2B Protein, His-tagged

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Cat.No. : UBE2B-531H
Product Overview : Recombinant Human UBE2B (Accession # NP_003328), fused with an N-terminal 6-His tag, was produced in E. coli.
Source : E. coli
Species : Human
Tag : His
Form : X mg/ml (X µM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP
Bio-activity : Recombinant Human His6-HR6B/UBE2B is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-HR6B/UBE2B concentration of 0.1-1 µM.
Molecular Mass : Recombinant Human UBE2B, His-tagged has a calculated MW of 19 kDa.
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name : UBE2B ubiquitin-conjugating enzyme E2B [ Homo sapiens ]
Official Symbol : UBE2B
Synonyms : UBE2B; ubiquitin-conjugating enzyme E2B; ubiquitin conjugating enzyme E2B (RAD6 homolog); ubiquitin-conjugating enzyme E2 B; HHR6B; RAD6B; UBC2; E2 protein; RAD6 homolog B; ubiquitin-protein ligase B; ubiquitin carrier protein B; ubiquitin-conjugating enzyme E2-17 kDa; ubiquitin-conjugating enzyme E2B (RAD6 homolog); HR6B; E2-17kDa;
Gene ID : 7320
mRNA Refseq : NM_003337
Protein Refseq : NP_003328
MIM : 179095
UniProt ID : P63146
Chromosome Location : 5q31.1

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