Active Recombinant Human WNT10B

• Cat.No.: WNT10B-534H
• Product Overview: Recombinant Human WNT10B (Accession # NP_003385) Met1-Lys389, fused with , was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Source: CHO Cells
• Species: Human
• Predicted N Terminal: Asn29
• Form: Lyophilized from a 0.2 µm filtered solution in PBS, EDTA and CHAPS with BSA as a carrier protein.
• Bio-activity: Measured in a cell proliferation/survival assay using IEC-18 rat small intestinal epithelial cells.The ED50 for this effect is typically 0.3 - 1.2 µg/mL.
• Molecular Mass: Recombinant Human WNT10B has a calculated MW of 40 kDa. In SDS-PAGE migrates as 41 kDa, reducing conditions.
• Protein length: Met1-Lys389
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: WNT10B wingless-type MMTV integration site family, member 10B [ Homo sapiens ]
• Official Symbol: WNT10B
• Synonyms: WNT10B; wingless-type MMTV integration site family, member 10B; protein Wnt-10b; SHFM6; WNT 12; WNT-10B protein; WNT-12
• Gene ID: 7480
• mRNA Refseq: NM_003394
• Protein Refseq: NP_003385
• MIM: 601906
• UniProt ID: O00744
• Chromosome Location: 12q13