Active Recombinant Human CDC25B Protein, Met & His-tagged

• Cat.No.: CDC25B-547H
• Product Overview: Recombinant Human CDC25B (Accession # NP_004349) Glu377-Gln566, fused with an N-terminal Met and 6-His tag, was produced in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: & His
• Predicted N Terminal: Met
• Form: Supplied as a 0.2 µm filtered solution in Tris, NaCl, EDTA, DTT and Glycerol.
• Bio-activity: Measured by hydrolysis of 250 µM 3-O-methyl fluorescein phosphate (OMFP).The specific activity is >0.5 µmol/min/mg, as measured under the described conditions.
• Molecular Mass: Recombinant Human CDC25B, Met & His-tagged has a calculated MW of 23 kDa. In SDS-PAGE migrates as 23-25 kDa, reducing conditions.
• Protein length: Glu377-Gln566
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: CDC25B cell division cycle 25 homolog B (S. pombe) [ Homo sapiens ]
• Official Symbol: CDC25B
• Synonyms: CDC25B; cell division cycle 25 homolog B (S. pombe); cell division cycle 25 homolog B (S. cerevisiae) , cell division cycle 25B; M-phase inducer phosphatase 2; cell division cycle 25B; dual specificity phosphatase Cdc25B
• Gene ID: 994
• mRNA Refseq: NM_004358
• Protein Refseq: NP_004349
• MIM: 116949
• UniProt ID: P30305
• Chromosome Location: 20p13