Recombinant Human DAG1 Protein, His-tagged DAG1-549H

Recombinant Human DAG1 Protein, His-tagged

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Recombinant Human DAG1 Protein, His-tagged

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Cat.No. : DAG1-549H
Product Overview : Recombinant Human DAG1 (Accession # NP_004384) Met1-Val749, fused with with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Source : NSO Cells
Species : Human
Tag : His
Predicted N Terminal : Gln28 predicted ( alpha subunit): No results obtained, sequencing might be blocked & Ser654 ( beta subunit)
Form : Lyophilized from a 0.2 µm filtered solution in PBS.
Bio-activity : Measured by the ability of the immobilized protein to enhance the adhesion of H4 human neuroglioma cells.The ED50 for this effect is typically 1.5 - 6.0 µg/mL. Optimal dilutions should be determined by each laboratory for each application.
Molecular Mass : Recombinant Human DAG1, His-tagged has a calculated MW of 67.8 kDa ( alpha subunit) & 11.3 kDa ( beta subunit). In SDS-PAGE migrates as 65-90 kDa & 20 kDa, reducing conditions.
Protein length : Met1-Val749
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution : Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Gene Name : DAG1 dystroglycan 1 (dystrophin-associated glycoprotein 1) [ Homo sapiens ]
Official Symbol : DAG1
Synonyms : DAG1; dystroglycan 1 (dystrophin-associated glycoprotein 1); dystroglycan; 156DAG; A3a; AGRNR; alpha dystroglycan; beta dystroglycan; DAG; dystrophin associated glycoprotein 1; MDDGC7; FLJ51254;
Gene ID : 1605
mRNA Refseq : NM_001165928
Protein Refseq : NP_001159400
MIM : 128239
UniProt ID : Q14118
Chromosome Location : 3p21

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