Recombinant Human DAG1 Protein, His-tagged


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Recombinant Human DAG1 Protein, His-tagged

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Cat.No. : DAG1-549H
Product Overview : Recombinant Human DAG1 (Accession # NP_004384) Met1-Val749, fused with with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Source : Mammalian cells
Species : Human
Tag : His
Predicted N Terminal : Gln28 predicted ( alpha subunit): No results obtained, sequencing might be blocked & Ser654 ( beta subunit)
Form : Lyophilized from a 0.2 µm filtered solution in PBS.
Bio-activity : Measured by the ability of the immobilized protein to enhance the adhesion of H4 human neuroglioma cells.The ED50 for this effect is typically 1.5 - 6.0 µg/mL. Optimal dilutions should be determined by each laboratory for each application.
Molecular Mass : Recombinant Human DAG1, His-tagged has a calculated MW of 67.8 kDa ( alpha subunit) & 11.3 kDa ( beta subunit). In SDS-PAGE migrates as 65-90 kDa & 20 kDa, reducing conditions.
Protein length : Met1-Val749
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution : Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Gene Name : DAG1 dystroglycan 1 (dystrophin-associated glycoprotein 1) [ Homo sapiens ]
Official Symbol : DAG1
Synonyms : DAG1; dystroglycan 1 (dystrophin-associated glycoprotein 1); dystroglycan; 156DAG; A3a; AGRNR; alpha dystroglycan; beta dystroglycan; DAG; dystrophin associated glycoprotein 1; MDDGC7; FLJ51254;
Gene ID : 1605
mRNA Refseq : NM_001165928
Protein Refseq : NP_001159400
MIM : 128239
UniProt ID : Q14118
Chromosome Location : 3p21
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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