Active Recombinant Human DAG1 Protein, His-tagged

• Cat.No.: DAG1-549H
• Product Overview: Recombinant Human DAG1 (Accession # NP_004384) Met1-Val749, fused with with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: Gln28 predicted ( alpha subunit): No results obtained, sequencing might be blocked & Ser654 ( beta subunit)
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to enhance the adhesion of H4 human neuroglioma cells.The ED50 for this effect is typically 1.5 - 6.0 µg/mL. Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Human DAG1, His-tagged has a calculated MW of 67.8 kDa ( alpha subunit) & 11.3 kDa ( beta subunit). In SDS-PAGE migrates as 65-90 kDa & 20 kDa, reducing conditions.
• Protein length: Met1-Val749
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: DAG1 dystroglycan 1 (dystrophin-associated glycoprotein 1) [ Homo sapiens ]
• Official Symbol: DAG1
• Synonyms: DAG1; dystroglycan 1 (dystrophin-associated glycoprotein 1); dystroglycan; 156DAG; A3a; AGRNR; alpha dystroglycan; beta dystroglycan; DAG; dystrophin associated glycoprotein 1; MDDGC7; FLJ51254
• Gene ID: 1605
• mRNA Refseq: NM_001165928
• Protein Refseq: NP_001159400
• MIM: 128239
• UniProt ID: Q14118
• Chromosome Location: 3p21