Active Recombinant Human MEP1B Protein, His-tagged

• Cat.No.: MEP1B-562H
• Product Overview: Recombinant Human MEP1B (Accession # NP_005916) Leu21-Ser593 & Thr23-Ser593, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: Leu21 & Thr23
• Form: Supplied as a 0.2 µm filtered solution in Tris, NaCl and Glycerol.
• Bio-activity: Measured by its ability to cleave a fluorogenic peptide substrate, Mca-SEVNLDAEFRK(Dpn)RR-NH2.The specific activity is >350 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Human MEP1B, His-tagged has a calculated MW of 66 kDa. In SDS-PAGE migrates as 85 kDa, reducing conditions.
• Protein length: Leu21-Ser593 & Thr23-Ser593
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: MEP1B meprin A, beta [ Homo sapiens ]
• Official Symbol: MEP1B
• Synonyms: MEP1B; meprin A, beta; meprin A subunit beta; PPH beta; meprin B; endopeptidase-2; PABA peptide hydrolase; N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit beta; N-benzoyl-L-tyrosyl-p-amino-benzoic acid hydrolase beta subunit
• Gene ID: 4225
• mRNA Refseq: NM_005925
• Protein Refseq: NP_005916
• MIM: 600389
• UniProt ID: Q16820
• Chromosome Location: 18q12.2-q12.3