Active Recombinant Human ST3GAL2 Protein, His-tagged

• Cat.No.: ST3GAL2-576H
• Product Overview: Recombinant Human ST3GAL2 (Accession # NP_008858) Pro52-Gln350, fused with an N-terminal CD33 signal and a C-terminal 6-His tag, was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Source: CHO Cells
• Species: Human
• Tag: His
• Predicted N Terminal: Gly56
• Form: Supplied as a 0.2 µm filtered solution in Tris and NaCl.
• Bio-activity: Measured by its ability to transfer Neu5Ac from CMP-Neu5Ac to bovine B1-3 galactosyl-N-acetyl galactosamine.The specific activity is >250 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Human ST3GAL2, His-tagged has a calculated MW of 35 kDa. In SDS-PAGE migrates as 30-40 kDa, reducing conditions.
• Protein length: Pro52-Gln350
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: ST3GAL2 ST3 beta-galactoside alpha-2,3-sialyltransferase 2 [ Homo sapiens ]
• Official Symbol: ST3GAL2
• Synonyms: ST3GAL2; ST3 beta-galactoside alpha-2,3-sialyltransferase 2; sialyltransferase 4B (beta galactosidase alpha 2,3 sialytransferase) , SIAT4B; CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 2; ST3GalA.2; ST3GALII; SIAT4-B; ST3Gal II; alpha 2,3-ST 2; beta-galactoside alpha-2,3-sialytransferase; beta-galactoside alpha-2,3-sialyltransferase 2; Gal-beta-1,3-GalNAc-alpha-2,3-sialyltransferase; sialyltransferase 4B (beta-galactosidase alpha-2,3-sialytransferase); SIAT4B; Gal-NAc6S
• Gene ID: 6483
• mRNA Refseq: NM_006927
• Protein Refseq: NP_008858
• MIM: 607188
• UniProt ID: Q16842
• Chromosome Location: 16q22.3