Active Recombinant Human CA5B Protein, His-tagged

• Cat.No.: CA5B-577H
• Product Overview: Recombinant Human CA5B (Accession # NP_009151) Cys34-Pro317, fused with a C-terminal 10-His tag, was produced in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Predicted N Terminal: Cys34 & Ser35
• Form: Supplied as a 0.2 µm filtered solution in Tris and NaCl.
• Bio-activity: Measured by its esterase activity. The specific activity is >150 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Human CA5B, His-tagged has a calculated MW of 34 kDa. In SDS-PAGE migrates as 34 kDa, reducing conditions.
• Protein length: Cys34-Pro317
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: CA5B carbonic anhydrase VB, mitochondrial [ Homo sapiens ]
• Official Symbol: CA5B
• Synonyms: CA5B; carbonic anhydrase VB, mitochondrial; carbonic anhydrase 5B, mitochondrial; carbonic dehydratase; carbonate dehydratase VB; CA-VB; MGC39962
• Gene ID: 11238
• mRNA Refseq: NM_007220
• Protein Refseq: NP_009151
• MIM: 300230
• UniProt ID: Q9Y2D0
• Chromosome Location: Xp22.1