Active Recombinant Mouse Tgfbi Protein, His-tagged

• Cat.No.: Tgfbi-621M
• Product Overview: Recombinant Mouse Tgfbi (Accession # NP_033395) Gly24-His683, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: Gly24
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells.rm beta IG-H3, immobilized at 5 µg/mL (100 µL/well), can induce greater than 50% cell adhesion.
• Molecular Mass: Recombinant Mouse Tgfbi, His-tagged has a calculated MW of 73.1 kDa. In SDS-PAGE migrates as 70 kDa, reducing conditions.
• Protein length: Gly24-His683
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
• Publication:
  Targeted Expression of TGFBIp Peptides in Mouse and Human Tissue by MALDI-Mass Spectrometry Imaging (2021)

Gene Information

• Gene Name: Tgfbi transforming growth factor, beta induced [ Mus musculus ]
• Official Symbol: Tgfbi
• Synonyms: TGFBI; transforming growth factor, beta induced; transforming growth factor-beta-induced protein ig-h3; beta ig-h3; transforming growth factor, beta induced, 68 kDa; 68kDa; big-h3; Beta-ig; AI181842; AI747162; MGC150270
• Gene ID: 21810
• mRNA Refseq: NM_009369
• Protein Refseq: NP_033395