Recombinant Human SDC3 Protein, His-tagged SDC3-685H

Recombinant Human SDC3 Protein, His-tagged


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Recombinant Human SDC3 Protein, His-tagged

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Cat.No. : SDC3-685H
Product Overview : Recombinant Human SDC3 (Accession # NP_055469) Gln48-Lys383, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Source : NSO Cells
Species : Human
Tag : His
Predicted N Terminal : No results obtained: Gln48 predicted
Form : Lyophilized from a 0.2 µm filtered solution in PBS.
Bio-activity : Measured by the ability of the immobilized protein to inhibit the adhesion of Saos-2 human osteosarcoma cells to human Fibronectin.When 5 x 10^4 cells/well are added to human Syndecan-3 and human Fibronectin (0.5 µg/mL) coated plates, cell adhesion is blocked in a dose dependent manner after 1 hour at 37 °C. The ED50 for this effect is typically 2.5 - 10 µg/mL.
Molecular Mass : Recombinant Human SDC3, His-tagged has a calculated MW of 35.5 kDa. In SDS-PAGE migrates as 100-150 kDa, reducing conditions.
Protein length : Gln48-Lys383
Purity : >85%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name : SDC3 syndecan 3 [ Homo sapiens ]
Official Symbol : SDC3
Synonyms : SDC3; syndecan 3; syndecan 3 (N syndecan); syndecan-3; N syndecan; SYND3; syndecan proteoglycan 3; N-syndecan; syndecan neural type; SDCN;
Gene ID : 9672
mRNA Refseq : NM_014654
Protein Refseq : NP_055469
MIM : 186357
UniProt ID : O75056
Chromosome Location : 1pter-p22.3

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