Active Recombinant Human UFM1 Protein, His-tagged

• Cat.No.: UFM1-701H
• Product Overview: Recombinant Human UFM1 (Accession # NP_057701), fused with an N-terminal 6-His tag, was produced in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Form: Supplied as a solution in HEPES, NaCl, DTT and Glycerol.
• Bio-activity: Recombinant Human His6-Pro UFM1 can be conjugated to substrate proteins via the subsequent actions of a UFM1-activating (E1) enzyme, a UFM1-conjugating (E2) enzyme, and a UFM1 ligase (E3). Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-Pro UFM1 concentration of 10-50 µM.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: UFM1 ubiquitin-fold modifier 1 [ Homo sapiens ]
• Official Symbol: UFM1
• Synonyms: UFM1; ubiquitin-fold modifier 1; C13orf20, chromosome 13 open reading frame 20; bA131P10.1; BM-002; C13orf20
• Gene ID: 51569
• mRNA Refseq: NM_016617
• Protein Refseq: NP_057701
• MIM: 610553
• UniProt ID: P61960
• Chromosome Location: 13q13.3