Recombinant Mouse Asah2 Protein, His-tagged Asah2-714M

Recombinant Mouse Asah2 Protein, His-tagged


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Recombinant Mouse Asah2 Protein, His-tagged

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Cat.No. : Asah2-714M
Product Overview : Recombinant Mouse Asah2 (Accession # NP_061300) Thr34-Thr756, fused with an N-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Source : NSO Cells
Species : Mouse
Tag : His
Predicted N Terminal : His
Form : Supplied as a 0.2 µm filtered solution in MES, NaCl and Sodium Cholate.
Bio-activity : Measured by its ability to hydrolyze the substrate C12:0 ceramide into sphingosine and dodecanoic acid. The specific activity is >3,000 pmol/min/µg, as measured under the described conditions.
Molecular Mass : Recombinant Mouse Asah2, His-tagged has a calculated MW of 81 kDa. In SDS-PAGE migrates as 113 kDa, reducing conditions.
Protein length : Thr34-Thr756
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution : Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Gene Name : Asah2 N-acylsphingosine amidohydrolase 2 [ Mus musculus ]
Official Symbol : Asah2
Synonyms : ASAH2; N-acylsphingosine amidohydrolase 2; neutral ceramidase; NCDase; N-CDase; acylsphingosine deacylase 2; neutral/alkaline ceramidase; AI585898;
Gene ID : 54447
mRNA Refseq : NM_018830
Protein Refseq : NP_061300

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