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Recombinant Mouse Asah2 Protein, His-tagged

Cat.No.: Asah2-714M
Product Overview: Recombinant Mouse Asah2 (Accession # NP_061300) Thr34-Thr756, fused with an N-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Source: NSO Cells
Species: Mouse
Tag: His
Predicted N Terminal: His
Form: Supplied as a 0.2 µm filtered solution in MES, NaCl and Sodium Cholate.
Bio-activity: Measured by its ability to hydrolyze the substrate C12:0 ceramide into sphingosine and dodecanoic acid. The specific activity is >3,000 pmol/min/µg, as measured under the described conditions.
Molecular Mass: Recombinant Mouse Asah2, His-tagged has a calculated MW of 81 kDa. In SDS-PAGE migrates as 113 kDa, reducing conditions.
Protein length: Thr34-Thr756
Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution: Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Gene Name: Asah2 N-acylsphingosine amidohydrolase 2 [ Mus musculus ]
Official Symbol: Asah2
Synonyms: ASAH2; N-acylsphingosine amidohydrolase 2; neutral ceramidase; NCDase; N-CDase; acylsphingosine deacylase 2; neutral/alkaline ceramidase; AI585898;
Gene ID: 54447
mRNA Refseq: NM_018830
Protein Refseq: NP_061300
MIM:

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