Active Recombinant Human ST14 Protein, Met & His-tagged

• Cat.No.: ST14-742H
• Product Overview: Recombinant Human ST14 (Accession # NP_068813) Gly596-Val855, fused with an N-terminal Met and 6-His tag, was produced in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: & His
• Predicted N Terminal: Val615
• Form: Supplied as a 0.2 µm filtered solution in Tris-HCl and Glycerol.
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate Boc-QAR-AMC.The specific activity is >10,000 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Human ST14, Met & His-tagged has a calculated MW of 26 kDa; Other fragments of 19 kDa, 17 kDa, 9 kDa and 6 kDa are also observed in the recombinant protein preparation.. In SDS-PAGE migrates as 27 kDa (major), 20 kDa, 17 kDa, 10 kDa, 6.5 kDa, reducing conditions.
• Protein length: Gly596-Val855
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: ST14 suppression of tumorigenicity 14 (colon carcinoma) [ Homo sapiens ]
• Official Symbol: ST14
• Synonyms: ST14; suppression of tumorigenicity 14 (colon carcinoma); PRSS14; suppressor of tumorigenicity 14 protein; epithin; HAI; matriptase; MT SP1; SNC19; TMPRSS14; prostamin; serine protease 14; serine protease TADG-15; membrane-type serine protease 1; tumor associated differentially expressed gene 15 protein; tumor-associated differentially-expressed gene 15 protein; suppression of tumorigenicity 14 (colon carcinoma, matriptase, epithin); MTSP1; MT-SP1; TADG15
• Gene ID: 6768
• mRNA Refseq: NM_021978
• Protein Refseq: NP_068813
• MIM: 606797
• UniProt ID: Q9Y5Y6
• Chromosome Location: 11q24-q25

Reviews

04/08/2022

Worked well in research.

07/14/2021

Great product, excellent quality.

02/10/2021

Reliable, high-quality product.

Q&As

How does ST14 contribute to the regulation of protease activity?

It regulates proteolytic activity, contributing to cellular processes like proliferation, differentiation, and migration.

In which cells or tissues is ST14 predominantly expressed?

ST14 is predominantly expressed in epithelial tissues and is also found in immune cells.

Are there known genetic mutations or alterations in ST14 associated with specific diseases?

Mutations and altered expression of ST14 have been associated with various types of cancers.

What role does ST14 play in the epidermal growth factor receptor (EGFR) signaling pathway?

ST14 activates the EGFR pathway by cleaving and activating pro-heparin-binding EGF-like growth factor (pro-HB-EGF).

What therapeutic strategies are being explored targeting ST14 for disease treatment?

Therapeutic strategies include developing ST14 inhibitors to prevent its pro-tumorigenic activities in cancer.

How is ST14 implicated in cancer progression and metastasis?

Overexpression of ST14 is linked to cancer progression, enhancing tumor cell invasion and metastasis.

What is the primary function of the ST14 protein in cellular processes?

ST14 (matriptase) is a type II transmembrane serine protease involved in the activation of other proteases and growth factors.