||Recombinant Rat Il12b&Il12a (Accession # NP_072133 (p40) & Q9R103 (p35)), fused with , was produced in Spodoptera frugiperda, Sf 21 (baculovirus)-derived.
||Baculovirus Sf21 Insect Cells
||Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
||Measured in a cell proliferation assay using PHA-activated mouse splenocytes. Mattner, F. et al. (1993) Eur. J. Immunol. 23:2202.The ED50 for this effect is typically 0.025 - 0.25 ng/mL.
||Recombinant Rat Il12b&Il12a has a calculated MW of 36.8 kDa (p40), 22.6 kDa (p35). In SDS-PAGE migrates as 42-45 kDa and 26-28 kDa, reducing conditions.
||>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
||Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
||Reconstitute at 10 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.