Recombinant Human WNT5B WNT5B-773H

Recombinant Human WNT5B

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Recombinant Human WNT5B

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Cat.No. : WNT5B-773H
Product Overview : Recombinant Human WNT5B (Accession # NP_110402) Gln18-Lys359, fused with , was produced in Chinese Hamster Ovary cell line, CHO-derived.
Source : CHO Cells
Species : Human
Predicted N Terminal : Gln18 predicted: No results obtained, sequencing might be blocked
Form : Lyophilized from a 0.2 µm filtered solution in PBS, EDTA and CHAPS with BSA as a carrier protein.
Bio-activity : Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells.The ED50 for this effect is typically 0.03-0.15 µg/mL, in the presence of 5 ng/mL of Recombinant Mouse Wnt-3a.Optimal dilutions should be determined by each laboratory for each application.
Molecular Mass : Recombinant Human WNT5B has a calculated MW of 38.5 kDa. In SDS-PAGE migrates as 45 kDa, reducing conditions.
Protein length : Gln18-Lys359
Purity : >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name : WNT5B wingless-type MMTV integration site family, member 5B [ Homo sapiens ]
Official Symbol : WNT5B
Synonyms : WNT5B; wingless-type MMTV integration site family, member 5B; protein Wnt-5b; WNT-5B protein; MGC2648;
Gene ID : 81029
mRNA Refseq : NM_030775
Protein Refseq : NP_110402
MIM : 606361
UniProt ID : Q9H1J7
Chromosome Location : 12p13.3

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