Active Recombinant Human WNT5B

• Cat.No.: WNT5B-773H
• Product Overview: Recombinant Human WNT5B (Accession # NP_110402) Gln18-Lys359, fused with , was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Source: CHO Cells
• Species: Human
• Predicted N Terminal: Gln18 predicted: No results obtained, sequencing might be blocked
• Form: Lyophilized from a 0.2 µm filtered solution in PBS, EDTA and CHAPS with BSA as a carrier protein.
• Bio-activity: Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells.The ED50 for this effect is typically 0.03-0.15 µg/mL, in the presence of 5 ng/mL of Recombinant Mouse Wnt-3a.Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Human WNT5B has a calculated MW of 38.5 kDa. In SDS-PAGE migrates as 45 kDa, reducing conditions.
• Protein length: Gln18-Lys359
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: WNT5B wingless-type MMTV integration site family, member 5B [ Homo sapiens ]
• Official Symbol: WNT5B
• Synonyms: WNT5B; wingless-type MMTV integration site family, member 5B; protein Wnt-5b; WNT-5B protein; MGC2648
• Gene ID: 81029
• mRNA Refseq: NM_030775
• Protein Refseq: NP_110402
• MIM: 606361
• UniProt ID: Q9H1J7
• Chromosome Location: 12p13.3