Active Recombinant Human CFHR5 Protein, His-tagged

• Cat.No.: CFHR5-774H
• Product Overview: Recombinant Human CFHR5 (Accession # NP_110414) Glu19-Glu569, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Protein Length: Glu19-Glu569
• Predicted N Terminal: Glu19
• Form: Lyophilized from a 0.2 µm filtered solution in Tris and NaCl.
• Bio-activity: Measured by its ability to inhibit the binding of biotinylated Recombinant Human Complement Factor H-related 5/CFHR5 to immobilized Recombinant Human C-Reactive Protein/CRP. McRae, J.L. et al. (2005) J. Immunol. 174:6250.Immobilized rhFGF R4/Fc Chimera at 5 µg/mL (100 µL/well) can bind rhFGF-12 with a linear range of 1.6 - 100 ng/mL.
• Molecular Mass: Recombinant Human CFHR5, His-tagged has a calculated MW of 64 kDa. In SDS-PAGE migrates as 73 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: CFHR5 complement factor H-related 5 [ Homo sapiens (human) ]
• Official Symbol: CFHR5
• Synonyms: FHR5; CFHL5; FHR-5; CFHR5D; complement factor H-related protein 5; complement factor H-related protein 5; factor H-related protein 5
• Gene ID: 81494
• mRNA Refseq: NM_030787.3
• Protein Refseq: NP_110414.1
• MIM: 608593
• UniProt ID: Q9BXR6
• Chromosome Location: 1q31.3