Active Recombinant Human SEMA6C Protein, Fc Chimera

• Cat.No.: SEMA6C-775H
• Product Overview: Recombinant Human SEMA6C (Accession # NP_112175), fused with , was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: Fc
• Predicted N Terminal: Ala25
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by its ability to cause collapse of chick embryonic dorsal root ganglia (DRG) neuron growth cones.The concentration of 5.0 - 10 µg/mL in the presence of 20 ng/mL of rhNT-3 is sufficient to cause significant growth cone collapse.Optimal concentrations should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Human SEMA6C, Fc Chimera has a calculated MW of 89.2 kDa. In SDS-PAGE migrates as 90-100 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: SEMA6C sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6C [ Homo sapiens ]
• Official Symbol: SEMA6C
• Synonyms: SEMA6C; sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6C; semaphorin-6C; KIAA1869; m Sema Y2; sema Y; semaphorin-Y; SEMAY; m-SemaY; m-SemaY2
• Gene ID: 10500
• mRNA Refseq: NM_001178061
• Protein Refseq: NP_001171532
• MIM: 609294
• UniProt ID: Q9H3T2
• Chromosome Location: 1q21.2