Active Recombinant Human PCDHA4 Protein, His-tagged

• Cat.No.: PCDHA4-780H
• Product Overview: Recombinant Human PCDHA4 (Accession # NP_113688) Met1-Asn697, fused with a C-terminal 6-His tag, was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Source: CHO Cells
• Species: Human
• Tag: His
• Predicted N Terminal: No results obtained: Gln30 predicted
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of BCE C/D-1b bovine corneal endothelial cells.The ED50 for this effect is typically 0.025-0.1 µg/mL. Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Human PCDHA4, His-tagged has a calculated MW of 73.0 kDa. In SDS-PAGE migrates as 80-100 kDa, reducing conditions.
• Protein length: Met1-Asn697
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: PCDHA4 protocadherin alpha 4 [ Homo sapiens (human) ]
• Official Symbol: PCDHA4
• Synonyms: PCDHA4; CNR1; CNRN1; CRNR1; PCDH-ALPHA4; protocadherin alpha 4; protocadherin alpha-4; PCDH-alpha-4; KIAA0345-like 10; ortholog of mouse CNR1
• Gene ID: 56144
• mRNA Refseq: NM_018907
• Protein Refseq: NP_061730
• MIM: 606310
• UniProt ID: Q9UN74
• Chromosome Location: 5q31