Active Recombinant Human CFC1

• Cat.No.: CFC1-785H
• Product Overview: Recombinant Human CFC1 (Accession # NP_115934), fused with , was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Predicted N Terminal: Tyr26
• Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
• Bio-activity: Measured by its ability to cause heart reversals when injected into the right lateral plate of a population of Xenopus neurulae embryos. 80 ng per embryo typically induces 25 - 50% heart reversals.Measured by its ability to inhibit the cell growth of DU145 human prostate carcinoma cells. Miyazaki, H. et al. (2004) Oncogene 23:9326.The ED50 for this effect is typically 1.5 - 7.5 µg/mL.
• Molecular Mass: Recombinant Human CFC1 has a calculated MW of 17.4 kDa. In SDS-PAGE migrates as 24 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: CFC1 cripto, FRL-1, cryptic family 1 [ Homo sapiens ]
• Official Symbol: CFC1
• Synonyms: CFC1; cripto, FRL-1, cryptic family 1; cryptic protein; CRYPTIC; HTX2; cryptic family protein 1; CFC1B; DTGA2; FLJ77897; MGC133213
• Gene ID: 55997
• mRNA Refseq: NM_032545
• Protein Refseq: NP_115934
• MIM: 605194
• UniProt ID: P0CG37
• Chromosome Location: 2q21.2