Active Recombinant T. maritima TM0306 Protein, Met & His-tagged

• Cat.No.: TM0306-791T
• Product Overview: Recombinant T. maritima TM0306 (Accession # NP_228118) Ile2-Glu449, fused with an N-terminal Met and 6-His tag, was produced in E. coli.

Specification

• Species: T.maritima
• Source: E.coli
• Tag: His
• Protein Length: Ile2-Glu449
• Predicted N Terminal: Met
• Form: Supplied as a 0.2 µm filtered solution in Tris and NaCl.
• Bio-activity: Measured by its ability to cleave a fluorogenic substrate 4-methylumbelliferyl-alpha -L-fucopyranoside.The specific activity is >1,600 pmol/min/µg, as measured under the described conditions. See Activity Assay Protocol on www.RnDSystems.com.
• Molecular Mass: Recombinant T. maritima TM0306, Met & His-tagged has a calculated MW of 53 kDa. In SDS-PAGE migrates as 43-50 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 ug/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: TM0306 alpha-L-fucosidase [ Thermotoga maritima MSB8 ]
• Official Symbol: TM0306
• Synonyms: TM0306; alpha-L-fucosidase
• Gene ID: 897238
• Protein Refseq: NP_228118.1