Active Recombinant E. coli degP Protein, His-tagged

• Cat.No.: degP-792E
• Product Overview: Recombinant E. coli degP (Accession # NP_414703) Ala27-Gln474, fused with a C-terminal 10 His tag and ERSIGS sequence, was produced in E. coli.

Specification

• Source: E. coli
• Species: E. coli
• Tag: His
• Predicted N Terminal: Ala27
• Form: Supplied as a 0.2 µm filtered solution in HEPES and NaCl.
• Bio-activity: Measured by its ability to cleave beta -casein.Recombinant E. coli HTRA/Protease Do will cleave >95% cleavage of beta -casein, as measured under the described conditions.
• Molecular Mass: Recombinant E. coli degP, His-tagged has a calculated MW of 49 kDa. In SDS-PAGE migrates as 50 kDa, 41 kDa and 27 kDa, reducing conditions.
• Protein length: Ala27-Gln474
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).