Recombinant Escherichia coli DEGP Protein (27-474 aa), His-tagged

• Cat.No.: DEGP-1568E
• Product Overview: Recombinant Escherichia coli (strain K12) DEGP Protein (27-474 aa) is produced by Yeast expression system. This protein is fused with a 6xHis tag at the N-terminal. Protein Description: Full Length of Mature Protein.

Specification

• Description: DegP acts as a chaperone at low tperatures but switches to a peptidase (heat shock protein) at higher tperatures. It degrades transiently denatured and unfolded proteins which accumulate in the periplasm following heat shock or other stress conditions. DegP is efficient with Val-Xaa and Ile-Xaa peptide bonds, suggesting a preference for beta-branched side chain amino acids. Only unfolded proteins devoid of disulfide bonds appear capable of being cleaved, thereby preventing non-specific proteolysis of folded proteins. Its proteolytic activity is essential for the survival of cells at elevated tperatures. It can degrade IciA, ada, casein, globin and PapA. DegP shares specificity with DegQ. DegP is also involved in the biogenesis of partially folded outer-mbrane proteins (OMP).
• Source: Yeast
• Species: E. coli
• Tag: His
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 48.8 kDa
• Protein length: 27-474 aa
• AA Sequence: AETSSATTAQQMPSLAPMLEKVMPS VVSINVEGSTTVNTPRMPRNFQQFF GDDSPFCQEGSPFQSSPFCQGGQGG NGGGQQQKFMALGSGVIIDADKGYV VTNNHVVDNATVIKVQLSDGRKFDA KMVGKDPRSDIALIQIQNPKNLTAI KMADSDALRVGDYTVAIGNPFGLGE TVTSGIVSALGRSGLNAENYENFIQ TDAAINRGNSGGALVNLNGELIGIN TAILAPDGGNIGIGFAIPSNMVKNL TSQMVEYGQVKRGELGIMGTELNSE LAKAMKVDAQRGAFVSQVLPNSSAA KAGIKAGDVITSLNGKPISSFAALR AQVGTMPVGSKLTLGLLRDGKQVNV NLELQQSSQNQVDSSSIFNGIEGAE MSNKGKDQGVVVNNVKTGTPAAQIG LKKGDVIIGANQQAVKNIAELRKVL DSKPSVLALNIQRGDSTIYLLMQ
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with reconstitution instruction is sent along with the products.

Gene Information

• Gene Name: degP periplasmic serine endoprotease DegP [ Escherichia coli str. K-12 substr. MG1655 ]
• Official Symbol: DEGP
• Synonyms: ECK0160; htrA; ptd
• Gene ID: 947139
• Protein Refseq: NP_414703
• UniProt ID: P0C0V0