Active Recombinant Human COL18A1

• Cat.No.: COL18A1-807H
• Product Overview: Recombinant Human COL18A1 (Accession # NP_569712) His1154-Ser1335, fused with an N-terminal Met and 8-His tag, was produced in E. coli.

Specification

• Source: E. coli
• Species: Human
• Predicted N Terminal: Met-His tag
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by its ability to inhibit the FGF basic-dependent proliferation of HUVEC human umbilical vein endothelial cells. Dhanabal, M. et al. (1999) Biochem. Biophys. Res. Commun. 258:345.The ED50 for this effect is typically 0.4-2 µg/mL in the presence of 20 µg/mL of Goat Anti-Human Endostatin Antigen Affinity-purified Polyclonal Antibody.
• Molecular Mass: Recombinant Human COL18A1 has a calculated MW of 21.6 kDa. In SDS-PAGE migrates as 21 kDa, reducing conditions.
• Protein length: His1154-Ser1335
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: COL18A1 collagen, type XVIII, alpha 1 [ Homo sapiens ]
• Official Symbol: COL18A1
• Synonyms: COL18A1; collagen, type XVIII, alpha 1; KNO, Knobloch syndrome, type 1; collagen alpha-1(XVIII) chain; endostatin; KNO1; KS; antiangiogenic agent; multi-functional protein MFP; KNO; FLJ27325; FLJ34914; MGC74745
• Gene ID: 80781
• mRNA Refseq: NM_030582
• Protein Refseq: NP_085059
• MIM: 120328
• UniProt ID: P39060
• Chromosome Location: 21q22.3