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Recombinant Human COL18A1

Cat.No.: COL18A1-807H
Product Overview: Recombinant Human COL18A1 (Accession # NP_569712) His1154-Ser1335, fused with an N-terminal Met and 8-His tag, was produced in E. coli.
Source: E. coli
Species: Human
Predicted N Terminal: Met-His tag
Form: Lyophilized from a 0.2 µm filtered solution in PBS.
Bio-activity: Measured by its ability to inhibit the FGF basic-dependent proliferation of HUVEC human umbilical vein endothelial cells. Dhanabal, M. et al. (1999) Biochem. Biophys. Res. Commun. 258:345.The ED50 for this effect is typically 0.4-2 µg/mL in the presence of 20 µg/mL of Goat Anti-Human Endostatin Antigen Affinity-purified Polyclonal Antibody.
Molecular Mass: Recombinant Human COL18A1 has a calculated MW of 21.6 kDa. In SDS-PAGE migrates as 21 kDa, reducing conditions.
Protein length: His1154-Ser1335
Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name: COL18A1 collagen, type XVIII, alpha 1 [ Homo sapiens ]
Official Symbol: COL18A1
Synonyms: COL18A1; collagen, type XVIII, alpha 1; KNO, Knobloch syndrome, type 1; collagen alpha-1(XVIII) chain; endostatin; KNO1; KS; antiangiogenic agent; multi-functional protein MFP; KNO; FLJ27325; FLJ34914; MGC74745;
Gene ID: 80781
mRNA Refseq: NM_030582
Protein Refseq: NP_085059
MIM: 120328
UniProt ID: P39060
Chromosome Location: 21q22.3

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