Recombinant Human DNER Protein, Fc Chimera DNER-821H

Recombinant Human DNER Protein, Fc Chimera

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Recombinant Human DNER Protein, Fc Chimera

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Cat.No. : DNER-821H
Product Overview : Recombinant Human DNER (Accession # NP_620711), fused with , was produced in Mouse myeloma cell line, NS0-derived.
Source : NSO Cells
Species : Human
Tag : Fc
Predicted N Terminal : Arg29
Form : Lyophilized from a 0.2 µm filtered solution in PBS.
Bio-activity : Measured by its ability to inhibit Pleiotrophin-induced neurite outgrowth of E16-E18 rat embryonic cortical neurons.When 4 x 10^4 neurons/well are added to 96-well plate containing serial dilutions of recombinant human DNER Fc chimera and immobilized recombinant human Pleiotrophin/PTN (1 µg/mL), neurite outgrowth is significantly inhibited in a dose dependent manner. The ED50 for this effect is typically 0.4 - 1.6 µg/mL.
Molecular Mass : Recombinant Human DNER, Fc Chimera has a calculated MW of 90.9 kDa (monomer). In SDS-PAGE migrates as 135-160 kDa, reducing conditions.
Purity : >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name : DNER delta/notch-like EGF repeat containing [ Homo sapiens ]
Official Symbol : DNER
Synonyms : DNER; delta/notch-like EGF repeat containing; delta and Notch-like epidermal growth factor-related receptor; bet; UNQ26; H_NH0150O02.1; WUGSC:H_NH0150O02.1; delta-notch-like EGF repeat-containing transmembrane;
Gene ID : 92737
mRNA Refseq : NM_139072
Protein Refseq : NP_620711
MIM : 607299
UniProt ID : Q8NFT8
Chromosome Location : 2q36.3

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