Active Recombinant S. agalactiae SAG1197 Protein, Met & His-tagged

• Cat.No.: SAG1197-835S
• Product Overview: Recombinant S. agalactiae SAG1197 (Accession # NP_688206) Ser259-Ile1072, fused with an N-terminal Met and 6-His tag, was produced in E. coli.

Specification

• Species: S.agalactiae
• Source: E.coli
• Tag: His
• Protein Length: Ser259-Ile1072
• Predicted N Terminal: Met
• Form: Supplied as a 0.2 µm filtered solution in Tris and NaCl.
• Bio-activity: Measured by its ability to cleave the native substrate Hyaluronan.The specific activity is >75,000 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant S. agalactiae SAG1197, Met & His-tagged has a calculated MW of 93 kDa. In SDS-PAGE migrates as 86 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 10 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: SAG1197 hyaluronate lyase [ Streptococcus agalactiae 2603V/R ]
• Official Symbol: SAG1197
• Synonyms: SAG1197
• Gene ID: 1.014e+006
• Protein Refseq: NP_688206.1