Recombinant Human AMICA1 Protein, Fc Chimera AMICA1-839H

Recombinant Human AMICA1 Protein, Fc Chimera

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Recombinant Human AMICA1 Protein, Fc Chimera

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Cat.No. : AMICA1-839H
Product Overview : Recombinant Human AMICA1 (Accession # NP_694938), fused with , was produced in Mouse myeloma cell line, NS0-derived.
Source : NSO Cells
Species : Human
Tag : Fc
Predicted N Terminal : Leu10
Form : Lyophilized from a 0.2 µm filtered solution in PBS.
Bio-activity : Measured by the ability of the immobilized protein to support the adhesion of BT-20 human breast cancer cells.When 5 x 10^4 cells/well are added to rhAMICA/Fc Chimera coated plates (10 µg/mL, 100 µL/well), approximately 55% - 75% will adhere after 1 hour at 37° C. Similar results were obtained using HeLa, A549, and T84 cell-lines. Optimal dilutions should be determined by each laboratory for each application.
Molecular Mass : Recombinant Human AMICA1, Fc Chimera has a calculated MW of 55.5 kDa (monomer). In SDS-PAGE migrates as 65-70 kDa, reducing conditions.
Purity : >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution : Reconstitute at 0.2 mg/mL in sterile, deionized water.
Gene Name : AMICA1 adhesion molecule, interacts with CXADR antigen 1 [ Homo sapiens ]
Official Symbol : AMICA1
Synonyms : AMICA1; adhesion molecule, interacts with CXADR antigen 1; junctional adhesion molecule-like; AMICA; Gm638; adhesion molecule AMICA; junctional adhesion molecule; dendritic cell-specific protein CREA7-1; dendritic-cell specific protein CREA7-1; dendritic-cell specific protein CREA7-4; adhesion molecule interacting with CXADR antigen 1; adhesion molecule that interacts with CXADR antigen 1; JAML; CREA7-1; CREA7-4; FLJ37080; MGC118814; MGC118815;
Gene ID : 120425
mRNA Refseq : NM_001098526
Protein Refseq : NP_001091996
MIM : 609770
UniProt ID : Q86YT9
Chromosome Location : 11q23.3

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