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Recombinant Human PRTG Protein, His-tagged

Cat.No.: PRTG-856H
Product Overview: Recombinant Human PRTG (Accession # NP_776175) Phe36-Ala952, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Source: NSO Cells
Species: Human
Tag: His
Predicted N Terminal: Phe36
Form: Lyophilized from a 0.2 µm filtered solution in PBS.
Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of P19 mouse embryonal carcinoma cells.When 5 x 10^4 cells per well are added to Recombinant Human Protogenin coated plates, cell adhesion is enhanced in a dose dependent manner. The ED50 for this effect is typically 2 - 8 µg/mL. Optimal dilutions should be determined by each laboratory for each application.
Molecular Mass: Recombinant Human PRTG, His-tagged has a calculated MW of 102.7 kDa. In SDS-PAGE migrates as 120-130 kDa, reducing conditions.
Protein length: Phe36-Ala952
Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name: PRTG protogenin [ Homo sapiens ]
Official Symbol: PRTG
Synonyms: PRTG; protogenin; protogenin homolog (Gallus gallus); FLJ25756; IGDCC5; immunoglobulin superfamily; DCC subclass; member 5; shen-dan; protein Shen-Dan; immunoglobulin superfamily, DCC subclass, member 5; FLJ12795; FLJ13221;
Gene ID: 283659
mRNA Refseq: NM_173814
Protein Refseq: NP_776175
MIM: 613261
UniProt ID: Q2VWP7
Chromosome Location: 15q21.3

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