Active Recombinant Human PRTG Protein, His-tagged

• Cat.No.: PRTG-856H
• Product Overview: Recombinant Human PRTG (Accession # NP_776175) Phe36-Ala952, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Protein Length: Phe36-Ala952
• Predicted N Terminal: Phe36
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of P19 mouse embryonal carcinoma cells.When 5 x 10^4 cells per well are added to Recombinant Human Protogenin coated plates, cell adhesion is enhanced in a dose dependent manner. The ED50 for this effect is typically 2 - 8 µg/mL. Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Human PRTG, His-tagged has a calculated MW of 102.7 kDa. In SDS-PAGE migrates as 120-130 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: PRTG protogenin [ Homo sapiens ]
• Official Symbol: PRTG
• Synonyms: PRTG; protogenin; protogenin homolog (Gallus gallus); FLJ25756; IGDCC5; immunoglobulin superfamily; DCC subclass; member 5; shen-dan; protein Shen-Dan; immunoglobulin superfamily, DCC subclass, member 5; FLJ12795; FLJ13221
• Gene ID: 283659
• mRNA Refseq: NM_173814
• Protein Refseq: NP_776175
• MIM: 613261
• UniProt ID: Q2VWP7
• Chromosome Location: 15q21.3