Active Recombinant Human PPM1A Protein, His-tagged

• Cat.No.: PPM1A-862H
• Product Overview: Recombinant Human PPM1A (Accession # NP_808820) Gly2-Lys324, fused with a C-terminal 6-His tag, was produced in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Predicted N Terminal: Gly2
• Form: Supplied as a 0.2 µm filtered solution in HEPES, NaCl, MnCl2 and DTT.
• Bio-activity: Measured by its ability to dephosphorylate the peptide substrate, DLDVPIPGRFDRRVS(PO3)VAAE.The specific activity is >400 nmol/min/mg, as measured under the described conditions.
• Molecular Mass: Recombinant Human PPM1A, His-tagged has a calculated MW of 37 kDa. In SDS-PAGE migrates as 41 kDa, reducing conditions.
• Protein length: Gly2-Lys324
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: PPM1A protein phosphatase, Mg2+/Mn2+ dependent, 1A [ Homo sapiens ]
• Official Symbol: PPM1A
• Synonyms: PPM1A; protein phosphatase, Mg2+/Mn2+ dependent, 1A; protein phosphatase 1A (formerly 2C), magnesium dependent, alpha isoform; protein phosphatase 1A; MGC9201; phosphatase 2C alpha; PP2CA; PP2Calpha; protein phosphatase 2C; alpha isoform; protein phosphatase 1A (formerly 2C), magnesium-dependent, alpha isoform; PP2C-ALPHA; FLJ42306
• Gene ID: 5494
• mRNA Refseq: NM_021003
• Protein Refseq: NP_066283
• MIM: 606108
• UniProt ID: P35813
• Chromosome Location: 14q23.1