Recombinant Human RSPO2 Protein, His-tagged RSPO2-866H

Recombinant Human RSPO2 Protein, His-tagged


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Recombinant Human RSPO2 Protein, His-tagged

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Cat.No. : RSPO2-866H
Product Overview : Recombinant Human RSPO2 (Accession # NP_848660) Gln22-Gly205, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Source : NSO Cells
Species : Human
Tag : His
Predicted N Terminal : No results obtained: Gln22 predicted
Form : Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
Bio-activity : Measured by its ability to synergize with Recombinant Mouse Wnt-3a to stabilize beta -catenin protein levels in HEK293T human embryonic kidney cells as assessed with Western blot.This effect is detected at doses as low as 1 ng/mL in the presence of 20 ng/mL recombinant mouse Wnt-3a.Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells.The typical ED50 is 0.7-2.8 ng/mL in the presence of 5 ng/mL recombinant mouse Wnt-3a.
Molecular Mass : Recombinant Human RSPO2, His-tagged has a calculated MW of 22 kDa. In SDS-PAGE migrates as 25-29 kDa, reducing conditions.
Protein length : Gln22-Gly205
Purity : >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name : RSPO2 R-spondin 2 [ Homo sapiens ]
Official Symbol : RSPO2
Synonyms : RSPO2; R-spondin 2; R spondin 2 homolog (Xenopus laevis); R-spondin-2; MGC35555; R-spondin 2 homolog; roof plate-specific spondin-2; CRISTIN2; MGC43342;
Gene ID : 340419
mRNA Refseq : NM_178565
Protein Refseq : NP_848660
MIM : 610575
UniProt ID : Q6UXX9
Chromosome Location : 8q23.1

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