Active Recombinant Human WNT7A

• Cat.No.: WNT7A-898H
• Product Overview: Recombinant Human WNT7A (Accession # O00755) Leu32-Lys349, fused with , was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Source: CHO Cells
• Species: Human
• Predicted N Terminal: Leu32
• Form: Lyophilized from a 0.2 µm filtered solution in PBS, EDTA and CHAPS with BSA as a carrier protein.
• Bio-activity: Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells.The ED50 for this effect is typically 1-3 µg/mL in the presence of 5 ng/mL recombinant mouse Wnt-3a. Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Human WNT7A has a calculated MW of 35.5 kDa. In SDS-PAGE migrates as 40-65 kDa, reducing conditions.
• Protein length: Leu32-Lys349
• Purity: >70%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: WNT7A wingless-type MMTV integration site family, member 7A [ Homo sapiens ]
• Official Symbol: WNT7A
• Synonyms: WNT7A; wingless-type MMTV integration site family, member 7A; protein Wnt-7a; proto oncogene Wnt7a protein; proto-oncogene Wnt7a protein
• Gene ID: 7476
• mRNA Refseq: NM_004625
• Protein Refseq: NP_004616
• MIM: 601570
• UniProt ID: O00755
• Chromosome Location: 3p25