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Recombinant Human NCAN Protein, His-tagged

Cat.No.: NCAN-922H
Product Overview: Recombinant Human NCAN (Accession # O14594) Glu23-Cys1321, fused with a C-terminal 6-His tag, was produced in Chinese Hamster Ovary cell line, CHO-derived.
Source: CHO Cells
Species: Human
Tag: His
Predicted N Terminal: Glu23
Form: Lyophilized from a 0.2 µm filtered solution in PBS.
Bio-activity: Measured by the ability of the immobilized protein to enhance the adhesion of H4 human neuroglioma cells.When 5 x 10^4 cells per well are added to recombinant human Neurocan coated plates, cell adhesion is enhanced in a dose dependent manner. The ED50 for this effect is typically 50 - 200 ng/mL.
Molecular Mass: Recombinant Human NCAN, His-tagged has a calculated MW of 141.5 kDa. In SDS-PAGE migrates as 140-290 kDa, reducing conditions.
Protein length: Glu23-Cys1321
Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name: NCAN neurocan [ Homo sapiens ]
Official Symbol: NCAN
Synonyms: NCAN; neurocan; chondroitin sulfate proteoglycan 3 , CSPG3; neurocan core protein; neurocan proteoglycan; chondroitin sulfate proteoglycan 3 (neurocan); CSPG3; FLJ44681;
Gene ID: 1463
mRNA Refseq: NM_004386
Protein Refseq: NP_004377
MIM: 600826
UniProt ID: O14594
Chromosome Location: 19p12

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