Recombinant Human NCAN Protein, His-tagged NCAN-922H

Recombinant Human NCAN Protein, His-tagged

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Recombinant Human NCAN Protein, His-tagged

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Cat.No. : NCAN-922H
Product Overview : Recombinant Human NCAN (Accession # O14594) Glu23-Cys1321, fused with a C-terminal 6-His tag, was produced in Chinese Hamster Ovary cell line, CHO-derived.
Source : CHO Cells
Species : Human
Tag : His
Predicted N Terminal : Glu23
Form : Lyophilized from a 0.2 µm filtered solution in PBS.
Bio-activity : Measured by the ability of the immobilized protein to enhance the adhesion of H4 human neuroglioma cells.When 5 x 10^4 cells per well are added to recombinant human Neurocan coated plates, cell adhesion is enhanced in a dose dependent manner. The ED50 for this effect is typically 50 - 200 ng/mL.
Molecular Mass : Recombinant Human NCAN, His-tagged has a calculated MW of 141.5 kDa. In SDS-PAGE migrates as 140-290 kDa, reducing conditions.
Protein length : Glu23-Cys1321
Purity : >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name : NCAN neurocan [ Homo sapiens ]
Official Symbol : NCAN
Synonyms : NCAN; neurocan; chondroitin sulfate proteoglycan 3 , CSPG3; neurocan core protein; neurocan proteoglycan; chondroitin sulfate proteoglycan 3 (neurocan); CSPG3; FLJ44681;
Gene ID : 1463
mRNA Refseq : NM_004386
Protein Refseq : NP_004377
MIM : 600826
UniProt ID : O14594
Chromosome Location : 19p12

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