Active Recombinant Human NCAN Protein, His-tagged

• Cat.No.: NCAN-922H
• Product Overview: Recombinant Human NCAN (Accession # O14594) Glu23-Cys1321, fused with a C-terminal 6-His tag, was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Source: CHO Cells
• Species: Human
• Tag: His
• Predicted N Terminal: Glu23
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to enhance the adhesion of H4 human neuroglioma cells.When 5 x 10^4 cells per well are added to recombinant human Neurocan coated plates, cell adhesion is enhanced in a dose dependent manner. The ED50 for this effect is typically 50 - 200 ng/mL.
• Molecular Mass: Recombinant Human NCAN, His-tagged has a calculated MW of 141.5 kDa. In SDS-PAGE migrates as 140-290 kDa, reducing conditions.
• Protein length: Glu23-Cys1321
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: NCAN neurocan [ Homo sapiens ]
• Official Symbol: NCAN
• Synonyms: NCAN; neurocan; chondroitin sulfate proteoglycan 3 , CSPG3; neurocan core protein; neurocan proteoglycan; chondroitin sulfate proteoglycan 3 (neurocan); CSPG3; FLJ44681
• Gene ID: 1463
• mRNA Refseq: NM_004386
• Protein Refseq: NP_004377
• MIM: 600826
• UniProt ID: O14594
• Chromosome Location: 19p12