Recombinant Human NCAN Protein, His-tagged


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Recombinant Human NCAN Protein, His-tagged

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Cat.No. : NCAN-922H
Product Overview : Recombinant Human NCAN (Accession # O14594) Glu23-Cys1321, fused with a C-terminal 6-His tag, was produced in Chinese Hamster Ovary cell line, CHO-derived.
Source : CHO Cells
Species : Human
Tag : His
Predicted N Terminal : Glu23
Form : Lyophilized from a 0.2 µm filtered solution in PBS.
Bio-activity : Measured by the ability of the immobilized protein to enhance the adhesion of H4 human neuroglioma cells.When 5 x 10^4 cells per well are added to recombinant human Neurocan coated plates, cell adhesion is enhanced in a dose dependent manner. The ED50 for this effect is typically 50 - 200 ng/mL.
Molecular Mass : Recombinant Human NCAN, His-tagged has a calculated MW of 141.5 kDa. In SDS-PAGE migrates as 140-290 kDa, reducing conditions.
Protein length : Glu23-Cys1321
Purity : >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name : NCAN neurocan [ Homo sapiens ]
Official Symbol : NCAN
Synonyms : NCAN; neurocan; chondroitin sulfate proteoglycan 3 , CSPG3; neurocan core protein; neurocan proteoglycan; chondroitin sulfate proteoglycan 3 (neurocan); CSPG3; FLJ44681;
Gene ID : 1463
mRNA Refseq : NM_004386
Protein Refseq : NP_004377
MIM : 600826
UniProt ID : O14594
Chromosome Location : 19p12
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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