Recombinant Human casein kinase 2, beta polypeptide CSNK2B-23H

Recombinant Human casein kinase 2, beta polypeptide


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Recombinant Human casein kinase 2, beta polypeptide

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Cat. No. : CSNK2B-23H
Product Overview : Recombinant Human Casein Kinase 2 beta is a non-glycosylated polypeptide having a molecular mass of 24.9 kDa. Casein Kinase 2 is purified by proprietary chromatographic techniques.
Description : The Casein kinase 2 (EC is a serine/threonine-selective protein kinase that is a tetramer of two alpha subunits and two beta subunits. The alpha subunits have the catalytic kinase domain. Casein kinase 2 has been implicated in cell cycle control, DNA repair, regulation of the circadian rhythm and other cellular processes. Casein kinase 2 activity has been reported to be activated following Wnt signaling pathway activation. A Pertussis toxin-sensitive G protein and Disheveled appear to be an intermediary between Wnt-mediated activation of the Frizzled receptor and activation of casein kinase 2.
Source : Escherichia Coli.
Unit Definition : 1U = 1µmol/min at 37 degree C) using the synthetic peptide RRRDDDSDDD.
Physical Appearance : Sterile Filtered White lyophilized (freeze-dried) powder.
Purity : Greater than 95% as determined by SDS-PAGE.
Formulation : CK2 lyophilized from 25mM Tris-HCl, 500mM NaCl, 1mM DTT, 1mM EDTA, protease inhibitor cocktail, pH 8.5.
Solubility : Solubilize in distilled H2O or 50% glycerol.
Biological Activity : Specific activity > 1U/mg.
Storage : Lyophilized CK2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CK2 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
PDB rendering based on 1jwh. :

Gene Name : CSNK2B casein kinase 2, beta polypeptide [ Homo sapiens ]
Synonyms : CSNK2B; casein kinase 2, beta polypeptide; G5A; CK2B; CK2N; MGC138222; MGC138224; phosvitin; OTTHUMP00000029063; OTTHUMP00000029064; Casein kinase II beta subunit; alternative name: G5a, phosvitin; CSK2B; OTTHUMP00000029060; Phosvitin
Gene ID : 1460
mRNA Refseq : NM_001320
Protein Refseq : NP_001311
MIM : 115441
UniProt ID : P67870
Chromosome Location : 6p21-p12; 6p21.3
Pathway : Adherens junction; Tight junction; Wnt signaling pathway
Function : identical protein binding; protein domain specific binding; protein kinase regulator activity; protein serine/threonine kinase activity

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