Active Recombinant Human OGT protein, His-tagged

• Cat.No.: OGT-940H
• Product Overview: Recombinant Human OGT(Cys323-Glu1041) fused with His tag at C-terminal was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 323-1041 a.a.
• Predicted N Terminal: Cys323
• Form: Supplied as a 0.2 μm filtered solution in Tris, NaCl and TCEP.
• Bio-activity: Measured by its ability to transfer GlcNAc from UDP-GclNAc to peptide OGT substrate. The specific activity is >25 pmol/min/μg.
• Molecular Mass: Predicted Molecular Mass: 81 kDa;SDS-PAGE: 70 kDa, reducing conditions
• Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >80%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane
• Storage: Avoid repeated freeze-thaw cycles.6 months from date of receipt, -20 to -70 centigrade as supplied.3 months, -20 to -70 centigrade under sterile conditions after opening.

Gene Information

• Gene Name: OGT O-linked N-acetylglucosamine (GlcNAc) transferase [ Homo sapiens (human) ]
• Official Symbol: OGT
• Synonyms: OGT; HRNT1; O-GLCNAC; O-linked N-acetylglucosamine (GlcNAc) transferase; UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit; O-GlcNAc transferase p110 subunit; O-GlcNAc transferase subunit p110; O-linked N-acetylglucosamine transferase 110 kDa subunit; UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase; uridinediphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyl transferase; O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase); EC 2.4.1.255
• Gene ID: 8473
• mRNA Refseq: NM_181672
• Protein Refseq: NP_858058
• MIM: 300255
• UniProt ID: O15294
• Chromosome Location: Xq13
• Pathway: Other types of O-glycan biosynthesis
• Function: acetylglucosaminyltransferase activity; enzyme activator activity; contributes_to histone acetyltransferase activity (H4-K16 specific)

Q&As

Could you please tell me which of your Human O-GlcNAc Transferase (OGT) products are enzymatically active? Thank you.

The OGT-940H is a hot-selling enzymatically active protein. The specific activity is >25 pmol/min/μg which is measured by its ability to transfer GlcNAc from UDP-GclNAc to peptide OGT substrate.