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Active Recombinant Mouse Ebi3&Il27 protein, His-tagged

Cat.No. : Ebi3&Il27-952M
Product Overview : Recombinant Mouse Ebi3&Il27 protein(O35228 & Q8K3I6)(Met1-Pro228 & Phe29-Ser234), fused with His tag, was expressed in HEK293.
  • Specification
  • Gene Information
  • Related Products
Source : HEK293
Species : Mouse
Tag : His
Form : Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Bio-activity : 1. Measured by its binding ability in a functional ELISA. Immobilized mouse IL27-His at 10 μg/ml (100 μl/well) can bind human IL27RA-Fc, The EC50 of human IL27RA-Fc is 0.26-0.62 μg/ml.2. Measured in antiviral assay using HepG2 cells infected with vesicular stomatitisvirus (VSV). The ED50 for this effect is 2-10ng/mL.
Molecular Mass : The recombinant mouse IL27 comprises 442 amino acids and has a predicted molecular mass of 49.5 kDa. The apparent molecular mass of the protein is approximately 60 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Protein Length : Met1-Pro228 & Phe29-Ser234
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method
Purity : > 85 % as determined by SDS-PAGE
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name : Ebi3 Epstein-Barr virus induced gene 3 [ Mus musculus ]
Official Symbol : Ebi3
Synonyms : EBI3; Epstein-Barr virus induced gene 3; interleukin-27 subunit beta; IL-27B; IL-27 subunit beta; epstein-Barr virus-induced gene 3 protein homolog; EBI-3; IL-27;
Gene ID : 50498
mRNA Refseq : NM_015766
Protein Refseq : NP_056581

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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