Active Recombinant Human CHST10 Protein, His-tagged

• Cat.No.: CHST10-969H
• Product Overview: Recombinant Human CHST10 (Accession # O43529) Pro32-Asn356, fused with a C-terminal 6-His tag, was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Species: Human
• Source: CHO
• Tag: His
• Protein Length: Pro32-Asn356
• Predicted N Terminal: Pro32
• Form: Supplied as a 0.2 µm filtered solution in Tris and NaCl.
• Bio-activity: Measured by its ability to transfer sulfate from PAPS to phenolpthalein glucuronic acid.The specific activity is >350 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Human CHST10, His-tagged has a calculated MW of 39 kDa. In SDS-PAGE migrates as 55-75 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: CHST10 carbohydrate sulfotransferase 10 [ Homo sapiens (human) ]
• Official Symbol: CHST10
• Synonyms: HNK1ST; HNK-1ST; carbohydrate sulfotransferase 10; carbohydrate sulfotransferase 10; HNK-1 sulfotransferase; huHNK-1ST
• Gene ID: 9486
• mRNA Refseq: NM_004854.4
• Protein Refseq: NP_004845.1
• MIM: 606376
• UniProt ID: O43529
• Chromosome Location: 2q11.2