Recombinant Human FIGF Protein, Met & His-tagged FIGF-976H

Recombinant Human FIGF Protein, Met & His-tagged

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Recombinant Human FIGF Protein, Met & His-tagged

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Cat.No. : FIGF-976H
Product Overview : Recombinant Human FIGF (Accession # O43915) Phe93-Ser201, fused with an N-terminal Met and C-terminal 6-His tag, was produced in Spodoptera frugiperda, Sf 21 (baculovirus)-derived.
Source : Baculovirus Sf21 Insect Cells
Species : Human
Tag : & His
Predicted N Terminal : Met
Form : Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
Bio-activity : Measured in a cell proliferation assay using HMVEC human microvascular endothelial cells. Marconcini, L. et al. (1999) Proc. Natl. Acad. Sci. USA 96:9671. The ED50 for this effect is typically 0.1-0.3 µg/mL.Measured by its binding ability in a functional ELISA.Immobilized recombinant human Flt-4 Fc Chimera at 5 µg/mL (100 µL/well) binds Recombinant Human VEGF-D with an apparent KD <15 nM.
Molecular Mass : Recombinant Human FIGF, Met & His-tagged has a calculated MW of 13 kDa (monomer). In SDS-PAGE migrates as 17-20 kDa, reducing conditions.
Protein length : Phe93-Ser201
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name : FIGF c-fos induced growth factor (vascular endothelial growth factor D) [ Homo sapiens ]
Official Symbol : FIGF
Synonyms : FIGF; c-fos induced growth factor (vascular endothelial growth factor D); VEGFD; vascular endothelial growth factor D; VEGF D; VEGF-D;
Gene ID : 2277
mRNA Refseq : NM_004469
Protein Refseq : NP_004460
MIM : 300091
UniProt ID : O43915
Chromosome Location : Xp22.31

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