Active Recombinant Human FIGF Protein, Met & His-tagged

• Cat.No.: FIGF-976H
• Product Overview: Recombinant Human FIGF (Accession # O43915) Phe93-Ser201, fused with an N-terminal Met and C-terminal 6-His tag, was produced in Spodoptera frugiperda, Sf 21 (baculovirus)-derived.

Specification

• Source: Baculovirus Sf21 Insect Cells
• Species: Human
• Tag: & His
• Predicted N Terminal: Met
• Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
• Bio-activity: Measured in a cell proliferation assay using HMVEC human microvascular endothelial cells. Marconcini, L. et al. (1999) Proc. Natl. Acad. Sci. USA 96:9671. The ED50 for this effect is typically 0.1-0.3 µg/mL.Measured by its binding ability in a functional ELISA.Immobilized recombinant human Flt-4 Fc Chimera at 5 µg/mL (100 µL/well) binds Recombinant Human VEGF-D with an apparent KD <15 nM.
• Molecular Mass: Recombinant Human FIGF, Met & His-tagged has a calculated MW of 13 kDa (monomer). In SDS-PAGE migrates as 17-20 kDa, reducing conditions.
• Protein length: Phe93-Ser201
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: FIGF c-fos induced growth factor (vascular endothelial growth factor D) [ Homo sapiens ]
• Official Symbol: FIGF
• Synonyms: FIGF; c-fos induced growth factor (vascular endothelial growth factor D); VEGFD; vascular endothelial growth factor D; VEGF D; VEGF-D
• Gene ID: 2277
• mRNA Refseq: NM_004469
• Protein Refseq: NP_004460
• MIM: 300091
• UniProt ID: O43915
• Chromosome Location: Xp22.31