Active Recombinant Human ENTPD5 Protein, His-tagged

• Cat.No.: ENTPD5-1034H
• Product Overview: Recombinant Human ENTPD5 (Accession # O75356) Val21-His428 (major) & Ser46-His428 (minor), fused with a C-terminal 6-His tag, was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Species: Human
• Source: CHO
• Tag: His
• Protein Length: Val21-His428 (major) & Ser46-His428 (minor)
• Predicted N Terminal: Val21 & Ser46
• Form: Supplied as a 0.2 µm filtered solution in Tris, CaCl2, NaCl and Glycerol.
• Bio-activity: Measured by its ability to hydrolyze the 5"-phosphate groups from the substrate guanosine-5"-diphosphate (GDP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit.The specific activity is >7,000 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Human ENTPD5, His-tagged has a calculated MW of 46 kDa. In SDS-PAGE migrates as 50-60 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: ENTPD5 ectonucleoside triphosphate diphosphohydrolase 5 [ Homo sapiens ]
• Official Symbol: ENTPD5
• Synonyms: ENTPD5; ectonucleoside triphosphate diphosphohydrolase 5; CD39L4, PCPH, proto oncogene CPH; NTPDase 5; ER-UDPase; CD39-like 4; GDPase ENTPD5; UDPase ENTPD5; proto-oncogene CPH; CD39 antigen-like 4; nucleoside diphosphatase; Pcph proto-oncogene protein; uridine-diphosphatase ENTPD5; guanosine-diphosphatase ENTPD5; PCPH; CD39L4; NTPDase-5; MGC163357; MGC163359
• Gene ID: 957
• mRNA Refseq: NM_001249
• Protein Refseq: NP_001240
• MIM: 603162
• UniProt ID: O75356
• Chromosome Location: 14q24