Active Recombinant Human NTN1

• Cat.No.: NTN1-1088H
• Product Overview: Recombinant Human NTN1 (Accession # O95631), fused with , was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Predicted N Terminal: Val22
• Form: Lyophilized from a 0.2 µm filtered solution in PBS and EDTA with BSA as a carrier protein.
• Bio-activity: Measured by its binding ability in a functional ELISA.When recombinant rat UNC5H2 Fc Chimera is immobilized at 5 µg/mL, recombinant human Netrin-1 binds with an apparent KD < 1 nM.Measured in a cell proliferation assay using RT4-D6P2T rat schwannoma cells.The ED50 for this effect is typically 0.5 - 2.0 µg/mL.
• Molecular Mass: Recombinant Human NTN1 has a calculated MW of 67.5 kDa. In SDS-PAGE migrates as 80-85 kda, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: NTN1 netrin 1 [ Homo sapiens ]
• Official Symbol: NTN1
• Synonyms: NTN1; netrin 1; netrin-1; Netrin 1; NTN1L; netrin 1, mouse, homolog of
• Gene ID: 9423
• mRNA Refseq: NM_004822
• Protein Refseq: NP_004813
• MIM: 601614
• UniProt ID: O95631
• Chromosome Location: 17p13-p12