|Product Overview:||Recombinant Human ALDH1A1(Ser2-Ser501) fused with an N-terminal Met and 6-His tag was expressed in E. coli.|
|Description:||Aldehyde dehydrogenases (ALDHs) are NAD(P)+-dependent enzymes that detoxify aldehydes by oxidizing them to carboxylic acids. Nineteen ALDHs are present in humans, expressed in a variety of organelles and having different substrate preferences. ALDH1A1 is a cytosolic enzyme that preferentially oxidizes retinaldehyde to retinoic acid. ALDH1A1 is expressed in the epithelium of many organs, including brain, liver, testis, eye lens and cornea. ALDH1A1 is highly expressed in brain dopaminergic neurons, where it produces the retinoic acid required for their differentiation and development. The retinoic acid produced by ALDH1A1 is also important for the differentiation of hematopoietic stem cells. ALDH1A1 is a major enzyme in the oxidation of acetaldehyde, a toxic metabolite of ethanol.|
|Predicted N Terminal:||Ser2|
|Form:||Supplied as a 0.2 μm filtered solution in Tris, NaCl, Glycerol and DTT.|
|Bio-activity:||Measured by its ability to produce NADH during the oxidation of propionaldehyde. The specific activity is >150 pmol/min/μg.|
|Molecular Mass:||Predicted Molecular Mass: 56 kDa
SDS-PAGE: 57 kDa, reducing conditions
|Endotoxin:||<1.0 EU per 1 μg of the protein by the LAL method.|
|Purity:||>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.|
|Storage:||Avoid repeated freeze-thaw cycles.
6 months from date of receipt, -70 centigrade as supplied.
3 months, -70 centigrade under sterile conditions after opening.
|Gene Name:||ALDH1A1 aldehyde dehydrogenase 1 family, member A1 [ Homo sapiens ]|
|Synonyms:||ALDH1A1; aldehyde dehydrogenase 1 family, member A1; ALDH1, PUMB1; retinal dehydrogenase 1; RALDH1; retinaldehyde dehydrogenase 1; ALHDII; RALDH 1; ALDH class 1; acetaldehyde dehydrogenase 1; aldehyde dehydrogenase 1, soluble; aldehyde dehydrogenase, liver cytosolic; ALDC; ALDH1; PUMB1; ALDH11; ALDH-E1; MGC2318;|
|Pathway:||Biological oxidations, organism-specific biosystem; Ethanol oxidation, organism-specific biosystem; Fatty Acid Omega Oxidation, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Phase 1 - Functionalization of compounds, organism-specific biosystem; Retinol metabolism, organism-specific biosystem;|
|Function:||Ras GTPase activator activity; aldehyde dehydrogenase (NAD) activity; androgen binding; oxidoreductase activity; retinal dehydrogenase activity;|