Active Recombinant Human CFD Protein, His-tagged

• Cat.No.: CFD-1106H
• Product Overview: Recombinant Human CFD (Accession # P00746) Ile26-Ala253, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: Ile26 & His35
• Form: Supplied as a 0.2 µm filtered solution in Tris, NaCl, CaCl2 and Glycerol.
• Bio-activity: Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl), in the presence of 5,5"Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468.The specific activity is >70 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Human CFD, His-tagged has a calculated MW of 26 kDa & 25 kDa. In SDS-PAGE migrates as 27 kDa & 26 kDa, reducing conditions.
• Protein length: Ile26-Ala253
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 200 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: CFD complement factor D (adipsin) [ Homo sapiens ]
• Official Symbol: CFD
• Synonyms: CFD; complement factor D (adipsin); D component of complement (adipsin) , DF, PFD, properdin factor D; complement factor D; ADN; properdin factor D; C3 convertase activator; complement factor D preproprotein; D component of complement (adipsin); DF; PFD; ADIPSIN
• Gene ID: 1675
• mRNA Refseq: NM_001928
• Protein Refseq: NP_001919
• MIM: 134350
• UniProt ID: P00746
• Chromosome Location: 19p13.3